How Deep to Dig?

Cellulases Dig Deep

Enzymatic hydrolysis of cellulose is key for the production of second generation biofuels, which represent a long-standing leading area in the field of sustainable energy. Despite the wealth of knowledge about cellulase structure and function, the elusive mechanism by which these enzymes disintegrate the complex structure of their insoluble substrate, which is the gist of cellulose saccharifica...

To dig or not to dig.

Time to dig deep into the plant proteome: a hunt for low-abundance proteins

Ravi Gupta, Yiming Wang, Ganesh K. Agrawal, Randeep Rakwal, Ick H. Jo, Kyong H. Bang and Sun T. Kim* 1 Plant Functional Genomics Laboratory, Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, South Korea 2 Plant Proteomics Group, Max Planck Institute for Plant Breeding Research, Cologne, Germany 3 Research Laboratory for Biotech...

Knowing how deep to sequence

up to 39 different combinations of stable isotopes can be encoded into lysine, metabolically incorporated into proteomes and decoded by high-resolution mass scans. This neutron encoding (NeuCode) SILAC method allows greater numbers of proteins to be quantified than does traditional SILAC. With expected future increases in instrument mass resolution, coupled with appropriate isotope-labeled reag...

The DIG Description Logic Interface: DIG/1.1

This document describes a simple API for a general description logic system.